DNA Vectors
Cayman Chemical TNFRSF8CD30 LNg Isoform EX 1mg
Source: Active recombinant C-terminal human IgG1 Fc-His-tagged CD30 expressed in HEK293 cells • Amino acids: 19-379 • MW: 66.5 kDa
IBA LifeSciences StrGate Acptr Vctr pCSG-IBAwt1
pCSG-IBAwt1 is a large expression vector with universal features for transient expression as well as for generation of stable mammalian cell lines. The vector carries an ampicillin resistance cassette for selection of transformed E. coli cells and ColE1 origin for a high plasmid copy number. Extrachromosomal replication in mammalian cells could occur either by origin of replication from Epstein-Barr Virus (oriP) or by SV40 ori. For the former the vector provides the EBNA-1 gene and for the latter the cell line has to be latently infected with SV40 or express the SV40 large T antigen (e.g. HEK293T, COS-1, COS-7). Stable cell lines can be selected by the neomycin resistance gene (NeoR). In addition, the human cytomegalovirus (CMV) immediate-early promoter enables a high-level expression in a wide range of mammalian cells. Besides to the direct cloning of the gene of interest into pCSG-IBA vectors with Esp3I, another option via a so-called entry vector is possible.
IBA LifeSciences StrGate Acptr Vctr pASG-IBA2
The pASG-IBA2 vector is designed for expression of recombinant proteins with a C-terminal Strep-tagII in E. coli. The vector carries an ampicillin resistance cassette for selection of transformed E. coli cells, F1 and ColE1 origin for a high plasmid copy number, the inducible tetracycline promoter/operator for the regulated expression of proteins, and the ompA signal for periplasmic secretion of the recombinant protein. Expression of the protein is induced by addition of anhydrotetracycline, which is a derivative of tetracycline that does not exhibit antibiotic activity. During the secretion into the periplasmic space the ompA signal sequence will be cleaved off. Please note that cloning into pASG-IBA Acceptor Vectors compulsorily requires the restriction enzyme Esp3I since no other MCS for the integration of a gene of interest is available. In addition to the direct cloning of the gene of interest into pASG-IBA vectors with Esp3I, another option via Entry Vector is possible.
IBA LifeSciences StrGate Acptr Vctr pASG-IBA3
The pASG-IBA3 vector is designed for expression of recombinant proteins with a C-terminal Strep-tagII in E. coli. The vector carries an ampicillin resistance cassette for selection of transformed E. coli cells, F1 and ColE1 origin for a high plasmid copy number, and the inducible tetracycline promoter/operator for the regulated expression of proteins. The vector can be combined with any E. coli strain since the tet-promoter works independently of the genetic background of E. coli. Expression of the recombinant protein is induced by addition of anhydrotetracycline, which is a derivative of tetracycline that does not exhibit antibiotic activity. Please note that cloning into pASG-IBA Acceptor Vectors compulsorily requires the restriction enzyme Esp3I since no other MCS for the integration of a gene of interest is available. In addition to the direct cloning of the gene of interest into pASG-IBA vectors with Esp3I, another option via a so-called Entry Vector is possible.
IBA LifeSciences StrGate Acptr Vctr pASG-IBA44
The pASG-IBA44 vector is designed for expression of recombinant proteins with an N-terminal Strep-tagII and a C-terminal His-tag in E. coli. The vector carries an ampicillin resistance cassette for selection of transformed E. coli cells, F1 and ColE1 origin for a high plasmid copy number, the inducible tetracycline promoter/operator for the regulated expression of proteins, and the ompA signal for periplasmic secretion of the recombinant protein. Expression of the recombinant protein is induced by addition of anhydrotetracycline, which is a derivative of tetracycline that does not exhibit antibiotic activity. During the secretion into the periplasmic space the ompA signal sequence will be cleaved off.
IBA LifeSciences StrGate Acptr Vctr pASG-IBA102
The pASG-IBA102 vector is designed for expression of recombinant proteins with a C-terminal Twin-Strep-tag in E. coli. The vector carries an ampicillin resistance cassette for selection of transformed E. coli cells, F1 and ColE1 origin for a high plasmid copy number, the inducible tetracycline promoter/operator for the regulated expression of proteins, and the ompA signal for periplasmic secretion of the recombinant protein. Expression of the recombinant protein is induced by addition of anhydrotetracycline, which is a derivative of tetracycline that does not exhibit antibiotic activity. During the secretion into the periplasmic space the ompA signal sequence will be cleaved off. In addition to the direct cloning of the gene of interest into pASG-IBA vectors with Esp3I, another option via a so-called Entry Vector is possible.
Cayman Chemical THUNDRTotal BTK TRFRET Ce 9600
Sandwich TR-FRET immunoassay for semi-quantitative detection of Total BTK (both phosphorylated and non-phosphorylated) in cell lysates
System Biosciences LLC PGREENFIRE1-LXRE IN LXR ALPHA
pGreenFire1-LXRE in LXR alpha (HepG2 cell line)
AB Vector LLC GC (Green Control), 1 mL
Positive control baculovirus in experiments where GFP fluorescence is used to monitor virus propagation, optimize cultivation conditions, or titrate of virus stocks.
IBA LifeSciences StrGate Acptr Vctr pASG-IBAwt2
The pASG-IBAwt2 vector is designed for expression of target proteins without affinity tag in E. coli. The vector carries an ampicillin resistance cassette for selection of transformed E. coli cells, F1 and ColE1 origin for a high plasmid copy number, the inducible tetracycline promoter/operator for the regulated expression of proteins, and the ompA signal for periplasmic secretion of the recombinant protein. Expression of the recombinant protein is induced by addition of anhydrotetracycline, which is a derivative of tetracycline that does not exhibit antibiotic activity. During the secretion into the periplasmic space the ompA signal sequence will be cleaved off. Please note that cloning into pASG-IBA Acceptor Vectors compulsorily requires the restriction enzyme Esp3I since no other MCS for the integration of a gene of interest is available. In addition to the direct cloning of the gene of interest into pASG-IBA vectors with Esp3I, another option via Entry Vector is possible.