DNA Vectors
BIOHIPPO AAV-CMV-EGFP-WPRE-hGH-polyA
Recombinant adeno associated virus expressing EGFP under the CMV promoter with WPRE and hGH polyA elements. Offered under the Biohippo AAVX Series with multiple serotypes available including AAV2/1, AAV2/2, AAV2/5, AAV2/6, AAV2/6m, AAV2/6.2FF, AAV2/7m8, AAV2/8, AAV2/9, AAV2/DJ, AAV2/DJ8, AAV2/Retro, AAV2/PHP.eB, AAV2/PHP.B, AAV2/rh10, AAV2/PHP.S, AAV2/anc80, AAV2/B10, AAV2/olig001, AAV2/BI30, AAV2/BR1, AAV2/MG1.2, AAV1/2, AAV2/MaCPNS1, and AAV2/MaCPNS2. Regular titer is greater than or equal to 2.00E+12 vg per mL, with high titer greater than or equal to 1.00E+13 vg per mL available upon request at no additional cost. Customers may specify preferred serotype and titer when placing orders.
BIOHIPPO AAV-RAM-d2tTA::TRE-ArchT-EGFP-WPRE-pA
Recombinant adeno associated virus expressing d2TTA ArchT fused to EGFP under the RAM TRE promoter with WPRE and hGH polyA elements. The Tet responsive configuration enables doxycycline controlled expression, providing precise targeted control of targeted neurons. Offered under the Biohippo AAVX Series with multiple serotypes available including AAV2/1, AAV2/2, AAV2/5, AAV2/6, AAV2/6m, AAV2/6.2FF, AAV2/7m8, AAV2/8, AAV2/9, AAV2/DJ, AAV2/DJ8, AAV2/Retro, AAV2/PHP.eB, AAV2/PHP.B, AAV2/rh10, AAV2/PHP.S, AAV2/anc80, AAV2/B10, AAV2/olig001, AAV2/BI30, AAV2/BR1, AAV2/MG1.2, AAV1/2, AAV2/MaCPNS1, and AAV2/MaCPNS2. Regular titer is greater than or equal to 2.00E+12 vg per mL, with high titer greater than or equal to 1.00E+13 vg per mL available upon request at no additional cost. Customers may specify preferred serotype and titer when placing orders.
New England Biolabs, Inc. PhiX174 RF II DNA – 150 µg
This commonly used cloning vector is the double-stranded nicked circular form of ΦX174 - relaxed form. Its molecular weight is 3.5 x10^6 Daltons and it is 5,386 base pairs in length. Greater then 90% of the molecules are RF II, the remainder are RF I (supercoiled) and linear ΦX174 DNA.
System Biosciences LLC PCDH-MSCV-MCS-EF1A-PURO CDNA D
pCDH-MSCV-MCS-EF1α-Puro cDNA Dual Promoter Cloning and Expression Lentivector
New England Biolabs, Inc. Lambda DNA (dam-) – 1250 µg
Duplex DNA is isolated from bacteriophage lambda (cI857ind 1 Sam 7). The bacteriophage is grown in an E. coli host which is deficient in adenine methylase (dam-). Therefore, those deoxyadenosine positions which are methylated in a wild type E. coli host are not methylated in the dam- host. This DNA gives complete digests with restriction endonucleases that are sensitive to dam methylation. Lambda DNA (dam-) is 48,502 base pairs in length.
New England Biolabs, Inc. PhiX174 Virion DNA – 250 µg
ΦX174 Virion DNA is the single-stranded viral DNA isolated from bacteriophage ΦX174 am3 cs70. It has a molecular weight of 1.70 x 10^6 daltons and is 5386 bases in length. Greater than 85% of the molecules are circular.
New England Biolabs, Inc. M13mp18 RF I DNA – 10 µg
M13mp18 is the double-stranded, covalently closed, circular form of DNA derived from bacteriophage M13. This phage vector contains single HindIII, SphI, SbfI, PstI, SalI (AccI/ HincII), XbaI, BamHI, SmaI (XmaI), KpnI (Acc65I), SacI and EcoRI sites within the -Galactosidase gene. When a fragment of DNA is inserted into one of these sites, the -Galactosidase gene is inactivated, providing selection for clones on the appropriate indicator plate.
New England Biolabs, Inc. M13K07 Helper Phage – 1.8 ml
M13KO7 Helper Phage is used in conjunction with the phagemid of choice. In the presence of a phagemid bearing a wild-type M13 or f1 origin, single-stranded phagemid DNA is packaged preferentially and secreted into the culture medium. This allows easy production of single-stranded phagemid DNA.
New England Biolabs, Inc. pTXB1 Vector – 10 µg
pTXB1 is an E. coli expression vector designed for the in-frame insertion of a target gene into the polylinker upstream of the Mxe intein/chitin binding domain. The fusion protein is bound to chitin beads and the thiol-induced cleavage activity of the intein releases the target protein.
ABclonal Technology mCherry-Tag Rabbit mAb
Protein tags are peptide sequences genetically grafted onto a recombinant protein. Often these tags are removable by chemical agents or by enzymatic means, such as proteolysis or intein splicing. Tags are attached to proteins for various purposes.Epitope tags are short peptide sequences which are chosen because high-affinity antibodies can be reliably produced in many different species. These are usually derived from viral genes, which explain their high immunoreactivity. Epitope tags include V5-tag, Myc-tag, HA-tag and NE-tag. These tags are particularly useful for western blotting, immunofluorescence and immunoprecipitation experiments, although they also find use in antibody purification.
